Bangladesh opener Naim Sheikh has gone back for 36 as Bangladesh are now reduced to 83-2.
Naim tried to break the shackles with a slog sweep off Washington Sundar, but only managed to find the fielder at deep mid-wicket.
Earlier, Naim put up a good stand with Liton of 59 for the opening stand.
India won the toss and their skipper Rohit Sharma invited Bangladesh to bat first.
Both sides have gone with the same eleven in this match.
Bangladesh beat India by seven wickets in the first match at Delhi to lead the series by 1-0.