The Bangladesh Cricket Board refused the Pakistan Cricket Board's offer to play the second and final Test match of the ongoing split series under lights.
Cricket website Cricinfo reported that the BCB that players are not ready to play day-night matches now.
"We have spoken with our team management and they are not ready to play a day-night Test at the moment, due to lack of preparation," Nizamuddin Chowdhury, the BCB chief executive, said that while talking to a local media outlet.
"The board understands their [team management] point of view and, after taking everything into consideration, we have decided not to play day-night Test in Pakistan."
The Bangladesh tour of Pakistan was divided into three parts after security concerns from BCB. The first phase, which included three T20Is, was played within four days of January in Lahore. And the first of two Tests were played earlier this month, Bangladesh losing by an innings and 44 runs.
The last phase to be played in April will feature a single ODI and the second Test, both in Karachi. The two-Test series is part of the World Test Championship.
Bangladesh played their maiden Test match against India and lost by an innings and 46 runs in Kolkata late last year.