The Standard Chartered has severed ties with the Duke of York's business mentoring initiative, Pitch@Palace.
Like accountancy firm KPMG, it pulled support from the scheme showing "commercial reasons", reports BBC.
Several businesses and universities are reviewing their association with Prince Andrew following a BBC interview about his links to convicted sex offender Jeffrey Epstein.
Sources have told the BBC the decisions by Standard Chartered and KPMG were made before the Newsnight interview.
Prince Andrew cancelled a planned visit to flood-hit areas of Yorkshire on Tuesday, three days after the interview aired, the Sun newspaper reported.
It is understood the visit was deemed inappropriate in the midst of an election campaign.
'Very serious questions'
Meanwhile, Boris Johnson and Jeremy Corbyn were asked about whether the duke was "fit for purpose" during their head-to-head debate on ITV on Tuesday evening.
The Labour leader said there were "very, very serious questions that must be answered and nobody should be above the law".
The prime minister said: "I think all our sympathies should be, obviously, with the victims of Jeffrey Epstein and the law must certainly take its course."
In his Newsnight interview, the Queen's third child said he still did not regret his friendship with US financier Epstein - who took his own life in August while awaiting trial on sex-trafficking charges in the US.
The interview has provoked a backlash, with businesses, charities and other institutions announcing that they were reviewing their association with the prince.
In addition to Standard Chartered and KPMG ending their support for Pitch@Palace: Pharmaceuticals company AstraZeneca and Hult International Business School are reviewing their partnerships with the business scheme
Outward Bound, the charity the Duke of Edinburgh was patron of for 65 years, has called a board meeting to discuss the prince's patronage
London Metropolitan University said it will consider the prince's role as its patron, saying it "opposes all forms of discrimination, abuse and human trafficking"
University of Huddersfield students are calling for the prince to be sacked as their chancellor
On Monday, the Huddersfield students' union panel passed a motion to lobby the prince to resign as their chancellor.
The university has since said that it listens to its students' views and will "now be consulting with them over the coming weeks".
The duke has stood by his decision to speak out, after critics labelled the interview a "car crash".
But speaking on the BBC's Victoria Derbyshire programme on Tuesday, Huddersfield student Tristan Smith criticised the prince over his friendship with Epstein.
He accused Prince Andrew of "trying to dismiss" the row and failing to recognise Epstein's victims.
Meanwhile, a woman who has accused Epstein of sexually abusing her as a 15-year-old has urged Prince Andrew to share information about his former friend.
The accuser, identified as "Jane Doe 15", did not accuse Prince Andrew of any wrongdoing but called on him and others to come forward and give a statement under oath.
Elsewhere, former home secretary Jacqui Smith alleged that Prince Andrew made racist comments to her during a state dinner.
"I have to say the conversation left us slack-jawed with the things that he felt it was appropriate to say," she told the LBC election podcast.
And Rohan Silva, who was an adviser to former prime minister David Cameron, also accused the prince of using a racial slur in his presence.
A Buckingham Palace spokesman strenuously denied the claims, adding that Prince Andrew "does not tolerate racism in any form".
There is no wholesale repudiation of Prince Andrew's public role.
But whether as a result of the interview he gave, or because of the continuing swirl of allegations, there is a falling away of support for the prince, both corporate and political.
The former Labour lord chancellor and justice secretary, Lord Falconer, told BBC Radio 4's PM programme that he thought the time had come for Prince Andrew to step away from public duties.
Those close to Prince Andrew say that a withdrawal from public life is not under consideration.
But if support continues to seep from him, it will undermine his public position.
There was also further reaction to the prince's BBC appearance.
Actress Rose McGowan - one of the most prominent figures of the #MeToo movement - told the Victoria Derbyshire programme she thought it was not a truthful interview.
"It's also certainly not the mark of someone who is an empathetic character who cares about victims in any way," she added.
The actress also said she wished more questions had been asked about Epstein's alleged victims.
"We can't forget there is human tragedy behind this... This has serious repercussions, serious ramifications and serious pain that is involved in this story."
However, Alastair Campbell - Tony Blair's ex-communications chief - said that although he thought the interview was a "mistake", it was not "as bad as it is now being defined".
Mr Campbell, who was another high-profile Briton to be named in Epstein's 97-page "black book" of contacts, also told the Today programme that he met the financier on a visit to the US for a funeral and found him to be "a bit creepy".
Prince Andrew's BBC interview followed allegations by Virginia Giuffre, known at the time as Virginia Roberts, who claims the prince had sex with her on three occasions - the first when she was aged 17.
Prince Andrew "categorically" denied having had sexual contact with her.
In an extraordinary interview, which you can watch in full on BBC iPlayer in the UK or YouTube elsewhere in the world, the duke said:
He had investigations carried out to establish whether a photograph of him with Ms Giuffre was faked, but they were inconclusive
He would testify under oath if "push came to shove" and his lawyers advised him to
He was unaware of an arrest warrant against Epstein when he invited him to Princess Beatrice's 18th birthday party at Windsor Castle
He does not regret his friendship with Epstein because of "the opportunities I was given to learn" from him about trade and business
Speaking out about his relationship with the financier had become almost "a mental health issue" for him.