A Sydney to Melbourne express train derailed near the Victorian town of Wallan, killing two people on Thursday, February 20.
According to emergency services, several other passengers were being treated for injuries, , reports the BBC.
One person was airlifted to a hospital in Melbourne.
Images showed a number of twisted carriages had left the track, with one on its side. It is unclear what caused the train to derail.
Paramedics from Ambulance Victoria said that "a large number of people are being assessed but are not believed to be seriously injured".
The train was reportedly carrying about 160 passengers when it left the tracks at 19:50 local time (08:50 GMT) on Thursday, some 45km (28 miles) north of Melbourne, the Victorian state capital.